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Questions on which microscope is best suited for your experiments? Contact Wendy Salmon

Light Microscopes

Widefield microscopy

Confocal Microscopy

Light Sheet Fluorescence Microscopy

  • Zeiss Lightsheet Z.1
    • 6 laser lines: 405nm, 440nm, 491nm, 515nm, 561nm, 640nm
    • Compatable with aqueous clearing solutions
    • Optics for imaging samples with refractive index of 1.33 (water) or 1.45 (CLARITY and similar clearing solutions).

Sample Preparation

Smart Clear II (not Pro) and Easy Gel CLARITY-based tissue clearing system (LifeCanvas Technologies)

LiveCell Stage-top Incubation Systems (Pathology Devices)

 

Imaging Software

Imaris (Bitplane) for 3D rendering and analysis. Tutorials and webinars.

Huygens (SVI) for deconvolution of image stacks. Tutorials and theory.

Fiji package of ImageJ for basic image handling and anaysis. Tutorials and info.

Volocity (Perkin Elmer) for 3D rendering and analysis. Online training.

 

Electron Microscopes & Sample Prep

As of August 22, 2019, the Keck Facility no longer provides service, support or training for the electron microscopy equipment. The equipment will remain on-site for already trained users. For those requiring training or service, we recommend contacting the Peterson (1957) Nanotechnology Materials Core Facility at MIT's Koch Center or the MIT MRSEC electron microscopy core.

FEI Technai Spirit Transmission Electron Microscope

Jeol 5600LV Scanning Electron Microscope

Ultramicrotomes

  • Leica Ultracut UC7 cuts 50-1000nm plastic sections
  • Leica Ultracut UCT equipped with an EM FCS cryo stage

Cryostat (Leica CM3050 S) cuts 2-200µm frozen sections

Critical Point Dryer (Tousimus Autosamdri-815)

Sputter Coating System (Hummer 6.2)

 

Updated 6/26/2016